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HPV16-E7 Antibody, Biotin Conjugated

Details

HPV16-E7 Antibody, Biotin Conjugated

Name

bs-10446R-Biotin

Catalog Number

333 EUR

Price

0.1ml

Size

More information

Antigen background

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Specificity

This is a highly specific antibody against HPV16-E7. This antibody may also detect HPV35-E7

Source

KLH conjugated synthetic peptide derived from middle of HPV16-E7

Long name

HPV16-E7 Polyclonal Antibody, Biotin Conjugated

Purification method

This antibody was purified via Protein A.

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

Synonyms

Protein E7; E7

Additional conjugation

Biotinylated

Concentration

1ug per 1ul

Modification

Unmodified

Clonality

Polyclonal

French translation

anticorps

Tested applications

WB, IHC-P

Immunogen_range

50-98/98

Target Antigen

HPV16-E7

Gene ID number

1489079

Swiss Prot

P03129

Conjugation

Biotin

Crossreactivity

Virus

Crossreactive species details

HPV16

Modification site

None

Excitation Emission

N/A

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