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rHPV Antibody, FITC Conjugated

Details

rHPV Antibody, FITC Conjugated

Name

bs-2212R-FITC

Catalog Number

333 EUR

Price

0.1ml

Size

More information

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Synonyms

CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF38; CXorf11; ZDHHC1; Palmitoyltransferase ZDHHC9; Zinc finger DHHC domain-containing protein 9; DHHC-9; Zinc finger protein 379; Zinc finger protein 38; ZDHHC9; CGI-89; UNQ261/PRO298

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Specificity

This is a highly specific antibody against rHPV.

Long name

rHPV Polyclonal Antibody, FITC Conjugated

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Additional conjugation

Anti-FITC Antibody

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

Modification

Unmodified

Clonality

Polyclonal

French translation

anticorps

Tested applications

IF(IHC-P)

Swiss Prot

Q9Y397

Crossreactivity

Virus

Gene ID number

51114

Source

rHPV

Modification site

None

Target Antigen

rHPV

Conjugation

FITC

Crossreactive species details

HPV

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