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HPV16-E7 Antibody, FITC Conjugated

Details

HPV16-E7 Antibody, FITC Conjugated

Name

bs-10446R-FITC

Catalog Number

333 EUR

Price

0.1ml

Size

More information

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Specificity

This is a highly specific antibody against HPV16-E7. This antibody may also detect HPV35-E7

Source

KLH conjugated synthetic peptide derived from middle of HPV16-E7

Long name

HPV16-E7 Polyclonal Antibody, FITC Conjugated

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Additional conjugation

Anti-FITC Antibody

Synonyms

Protein E7; E7

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

Modification

Unmodified

Clonality

Polyclonal

French translation

anticorps

Tested applications

IF(IHC-P)

Immunogen_range

50-98/98

Target Antigen

HPV16-E7

Gene ID number

1489079

Swiss Prot

P03129

Crossreactive species details

HPV16

Crossreactivity

Virus

Modification site

None

Conjugation

FITC

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