E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity).
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
E7 protein [Human papillomavirus type 33]; Human Papilloma Virus; Human papillomavirus type 33; Human papillomavirus type 33; Protein 33; early protein E7 [Human papillomavirus type 33].
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
This antibody will also detect E7 in HPV58, but with slightly less affinity, due to a one amino acid change in the immunogen sequence range.HPV33 E7
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
KLH conjugated synthetic peptide derived from HPV33 E7
No modification has been applied to this antibody
For facs or microscopy Alexa 1 conjugate.
Rabbit (Oryctolagus cuniculus)
Alexa Fluor,ALEXA FLUOR® 594
HPV33 E7 Polyclonal Antibody
Conjugated Primary Antibody
Polyclonals and antibodies
Anti-HPV33 E7 Polyclonal
Purified by Protein A.
Polyclonal Antibodies
Polyclonal Antibody
IF(IHC-P)(1:50-200)
ALEXA FLUOR® 594
Alexa conjugate 1
1ug per 1ul
590nm/617nm
IF(IHC-P)
HPV33 E7
Nucleus
HPV33
Virus
None
N/A
N/A