Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen(both types of stimulants are needed to induce IL-2 production.
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
For cells, cell lines and tissues in culture till half confluency.
Interleukin-2(Interleukin 2, Il-2), Cd3; Homo Sapiens, Expressed
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Receptor expression: T cell antigen receptor, expressed
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
CD3; Homo sapiens, expressed
Liquid nitrogen -196°C
Jurkat, Clone E6-1
Every 2 to 3 days