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HPV33 E7 Antibody, FITC Conjugated

Details

HPV33 E7 Antibody, FITC Conjugated

Name

bs-2969R-FITC

Catalog Number

333 EUR

Price

0.1ml

Size

More information

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity).

Specificity

This is a highly specific antibody against HPV33 E7. This antibody will also detect E7 in HPV58, but with slightly less affinity, due to a one amino acid change in the immunogen sequence range.

Synonyms

E7 protein [Human papillomavirus type 33]; Human Papilloma Virus; Human papillomavirus type 33; Human papillomavirus type 33; Protein 33; early protein E7 [Human papillomavirus type 33].

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Source

KLH conjugated synthetic peptide derived from HPV33 E7

Long name

HPV33 E7 Polyclonal Antibody, FITC Conjugated

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Additional conjugation

Anti-FITC Antibody

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

Clonality

Polyclonal

Modification

Unmodified

French translation

anticorps

Tested applications

IF(IHC-P)

Immunogen_range

30-80/97

Target Antigen

HPV33 E7

Subcellular location

Nucleus

Crossreactivity

Virus

Crossreactive species details

HPV33

Conjugation

FITC

Modification site

None

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