4

Mouse Monoclonal IgG Anti-HPV (Type 1, 6, 11, 16, 18, 31) Human Papilloma virus (Screening Antibody) [1H8]

Details

Mouse Monoclonal IgG Anti-HPV (Type 1, 6, 11, 16, 18, 31) Human Papilloma virus (Screening Antibody) [1H8]

Name

HP300

Catalog Number

262 EUR

Price

3 ml

Size

More information

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Properties

If you buy Antibodies supplied by bio logo they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Litterature

1. Cowsert et al. (1987) J. Natl. Cancer Inst. 79; 1053-1057. 2. Banks L.J. (1987) J. Gen. Virol. 68; 1351-1355. 3. Cason J., Patel D., Naylor J., et al. (1989) Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. J. General Virol. 70; 2973-2987. 4. Shepherd P.S., Lunny D., Brooks R., et al. (1992) The detection of human papilloma viruses in cervical biopsies by immunohistochemistry and in situ hybridization. Scand. J. Immunol. 36(11); 69-74.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002 if required.

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Pretreatment

temperature antigen retrieval using 0.01 M citrate retrieval solution (pH 6.0) is recommended.

Availability

Please contact us to check the availability

Immunogen

SDS-disrupted bovine papillomavirus type 1

Specificity

Papillomavirus Type 1, 6, 11, 16, 18, 31

Positive control

Infected cervical biopsy

UniProt number

Go to UniProt/SwissProt

Product category

Primary Antibody

Storage method

Store at 2-8°C

Latin name

Mus musculus

Product is supplied as

ready to use

Tested applications

IHC(C, P)

Virus

papilloma

French translation

anticorps

Antibody's raised in

Mouse

Recommended concentration for use

neat

Protein concentration

N/A

Antibody's clone

1H8

Additional isotype

IgG

Antibody's isotype

IgG

Back to top