TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
(Ⅰ) LightCycler 1.0 (Internal control can't be used for this system)(Ⅱ) LightCycler 2.0
PCR, polymerase chain reaction