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Human UBE3A (Ubiquitin Protein Ligase E3A) ELISA Kit

Details

Human UBE3A (Ubiquitin Protein Ligase E3A) ELISA Kit

Name

EKE60775

Catalog Number

759 EUR

Price

96 Tests

Size

More information

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UBE3A. The concentration of Human UBE3A in samples can be calculated by comparing the OD of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Shelf life

Use Human UBE3A (Ubiquitin Protein Ligase E3A) ELISA Kit before 6 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Cross Activity

No significant cross-reactivity or interference was observed.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Cell Biology, Epigenetics and Nuclear Signaling, Neuroscience

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Sample Type

Serum, Plasma, Biological Fluids

Estimated Turnaround Time

6-11 business days

Detection Range

0.31-20 ng/mL

Detection Method

Colorimetric

Sensitivity

0.19 ng/mL

Assay Time

4.5 hours

Shipping Conditions

Ice packs

Assay Type

Sandwich

Species Reactivity

Human

Target's alterntive name

UBE3A

Target Name

NA

Specificity

NA

Precision

NA

Stability

NA

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