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Anti-HPV16-E7 (Polyclonal), ALEXA Fluor 594

Details

Anti-HPV16-E7 (Polyclonal), ALEXA Fluor 594

Name

GENTObs-10446R-A594

Catalog Number

489 EUR

Price

100 microliters

Size

More information

Background information

Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Synonyms

E7; Human Papilloma Virus; Human papillomavirus type 16 E7; Human papillomavirus type 16 E7; Protein E7; Human Papillomavirus 16 E7; HPV16 E7; E7 protein HPV16; VE7_HPV16.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Antigen Source

KLH conjugated synthetic peptide derived from middle of HPV16-E7

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with HPV16-E7

Properties

For facs or microscopy Alexa 1 conjugate.

Host organism

Rabbit (Oryctolagus cuniculus)

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Also known as

HPV16-E7 Polyclonal Antibody

Type

Conjugated Primary Antibody

Group

Polyclonals and antibodies

Other name

Anti-HPV16-E7 Polyclonal

Purification method

Purified by Protein A.

Clone

Polyclonal Antibodies

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Applications

IF(IHC-P)

Target Protein/Peptide

HPV16-E7

Gene ID

1489079

Cross Reactive Species details

HPV16

Cross reactive species

Virus

Modification site

None

Subcellular locations

N/A

Swiss Prot

N/A

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