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Jurkat, Clone E6-1 Cell Line

Details

Jurkat, Clone E6-1 Cell Line

Name

EP-CL-0129

Catalog Number

344 EUR

Price

1×10⁶ cells/vial

Size

More information

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Background

Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen(both types of stimulants are needed to induce IL-2 production.

Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Description

For cells, cell lines and tissues in culture till half confluency.

Gene_expression

Interleukin-2(Interleukin 2, Il-2), Cd3; Homo Sapiens, Expressed

Medium

RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)

Receptor_expression

Receptor expression: T cell antigen receptor, expressed

Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Antigen_expression

CD3; Homo sapiens, expressed

Storage

Liquid nitrogen -196°C

Abbreviation

Jurkat, Clone E6-1

Renewal

Every 2 to 3 days

Tissue

Peripheral blood

Applications

Transfection.

Morphology

Lymphoblast

Growth_properties

Suspension

Leadtime

3-5 days

Shipping condition

Dry ice

Ratio

1:2-1:4

Tumorigenic

NA

Effects

NA

Doubling_time

NA

Duration

NA

Age

NA

Biosafety

1

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