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Human UBE3A(Ubiquitin Protein Ligase E3A) ELISA Kit

Details

Human UBE3A(Ubiquitin Protein Ligase E3A) ELISA Kit

Name

ELK1751

Catalog Number

608 EUR

Price

96T

Size

More information

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Alternative Names

ANCR; AS; E6-AP; EPVE6AP; HPVE6A; Papilloma Virus E6-Associated,Angelman Syndrome; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Enzyme & Kinase;Developmental science;

Detection range

0.312-20ng/mL

Sensitivity

0.119ng/mL

Assay Type

Sandwich

Standard

20ng/mL

Assay length

3h

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