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Mouse Anti-Human HSP40, Hdj1 Monoclonal IgG1, Clone 3B9.E6

Details

Mouse Anti-Human HSP40, Hdj1 Monoclonal IgG1, Clone 3B9.E6

Name

SMC-145D-ALP

Catalog Number

359 EUR

Price

100 µg

Size

More information

Scientific context

Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (SMC-145). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-145) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (SMC-145). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-145) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [3B9.E6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-145) Mouse Anti-Hsp40 Antibody [3B9.E6] used in Western Blot (WB) on Human Cell lysates (SMC-145)

Test

StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Bibliography

1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91. 4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890. 7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Representative figure link

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Antibody's other name

DNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Antibody certificate of analysis

0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antibody's dilutions

WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's full description

Mouse Anti-Human HSP40 Monoclonal IgG1 Antibody, Clone: 3B9.E6: Alkaline Phosphatase

Antibody's specificity

Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.

Tissue specificity

See included datasheet or contact our support service

Antibody buffer for storage

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Immunogen

Recombinant Protein HSP40 (Hdj1)

Antibody's datasheet

Contact our support service

Antibodies' applications

WB, IHC, ICC/IF, IP, ELISA

Antibody's category

Monoclonal Antibodies

Antibody in cell

Cytoplasm , Nucleus

Primary research fields

Cancer, Heat Shock

Purity

Protein G Purified

Antibody's reactivity

Human, Mouse, Rat

Shipping recommendations

Blue Ice or 4ºC

Warning information

Non-hazardous

Latin name

Mus musculus

Tested reactivity

To be tested

Tested applications

To be tested

NCBI number

NP_006136.1

Released date

1-Nov-2008

Stock availability

In Stock

Antibody's concentration

1 mg/ml

Clone

3B9.E6

Country of production

Canada

Storage recommendations

-20ºC

Protein number

P25685

Antibody's target

HSP40

Raised in

Mouse

Gene number

3337

Brandname

None

Additional isotype

IgG1

Net weight (g)

0.1

Total weight (kg)

1.4

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